• Twitter
  • LinkedIn
  • Contact
  • Login
(+34) 985 98 06 05 | info@nanovexbiotech.com
  • 0Shopping Cart
EN Nanovex Biotechnologies SL
  • Home
  • Services
    • Encapsulation
    • Characterization
    • Bioconjugation
    • Exosome characterization
  • Products
    • Pronanosomes
    • Nanoparticles
    • Transfectosomes
    • PLGA nanoparticles
    • SEC Columns
    • Practice kits
    • SYNTHETIC EXOSOME
  • Projects
  • News&Blog
    • News
    • Blog
  • Contact
  • About us
  • INDERMAL
  • Search
  • Menu Menu
You are here: Home1 / Products2 / SEC Columns3 / SEC5K – Purification Column

SEC5K – Purification Column

50,00€ tax NOT included

Clear
SKU: N/A Category: SEC Columns Tags: column, desalting columns, protein purification, purification column, sec, sec purification column
  • Description
  • Additional information
  • Reviews (0)

Description

SEC5K – Purification Column

The Nanovex-SEC5K allows quick and economical purifications (protein, nanovesicle or nucleic acid) with exclusion limits of 5,000 Daltons (components larger than 5,000 Daltons eluted with void volume). The Nanovex-SEC5K columns are shipped containing ultrapure water/ethanol (20% v/v ethanol) as a preservative.

Pretreatment

  1. Shake the column and its contents gently several times, in order to resuspend the resin. Then, place the column upright and allow settling the resin bed.
  2. Carefully remove the storage solution from the top of gel bed.
  3. Equilibrate the column by adding five gel bed volumes (50 ml) of the desired buffer.

Sample purification or desalting

  1. Carefully remove the buffer solution from the top of gel bed.
  2. Add the desired sample volume to the top of the column and collect the same volume of eluent (fraction 1). After the sample has entered into the column and the first fraction of eluate has been collected, add a volume of 1 ml of the buffer solution, and simultaneously collect the same volume of eluent from the bottom of the column (0.5 ml fractions) as they emerge from the column.
  3. Repeat this step until the desired number of fractions has been collected.
  4. Then analyze the collected fractions with the appropriate detector and plot the recorded signal against fraction number.

Applications

  • Desalting
  • Removal of free low molecular weight molecules from protein
  • Removal of free low molecular weight molecules from nanovesicles.

Example 1: Separation of nanovesicles containing fluorescein from unencapsulated fluorescein.

  1. Equilibrate the SEC column with five column volumes (50 ml) of PBS.
  2. Apply the reaction mixture (200 µl) to column. When the reaction mixture has entered into the column and the first fraction was collected, add PBS and continue collecting separate 1 ml fractions as they emerged from the column (Typically 30 fractions are collected).
  3. For detection of nanovesicles and unencapsulated fluoresceín, absorbance measurements at 500 nm wavelength of all fractions were carried out (Typically fractions 4 to 6 will contain the nanovesicles, see the figure below).

 

Example 2: Separation of protein modified with SATA from unincorporated SATA.

  1. Equilibrate the SEC column with five column volumes (50 ml) of PBS containing 10 mM EDTA.
  2. Apply the reaction mixture (1 ml) to column and collect 1 ml fraction. When the reaction mixture has entered into the column and the first fraction was collected, add PBS solution containing 10 mM EDTA and continue collecting separate 1 ml fractions as they emerged from the column (Typically 10 fractions are collected).
  3. Protein detection is performed by absorbance measurements at 280 nm of all fractions. (Typically fractions 4 to 6 will contain the protein, see the figure below).

Specifications

MatrixDextran
Column Volume12 ml
Sample sizeUp to 1 ml
Volume gel bed10 ml
Void volume˜3.3 ml
Bead diameter (wet)85-260 µm
pH stability2  to 13
Storage temperatureAmbient
Autoclavable121ºC, pH 7.0, 30 min
Chemical stabilityAll commonly used buffers, included:

0.1M NaOH

0.01M HCl

1M Acetic acid

8M Urea

24% Ethanol

30% Propanol

30% Acetonitrile

Storage

Store in a cool and dry place and protected from the light.

Additional information

Number of columns

5 x 12ml Nanovex SEC-5K column

Related products

  • Eviso – Exosome isolation columns

    92,00€ – 322,00€ tax NOT included
    Select options
Nanovex Biotechnologies SL is an innovative technology-based spin-off from the University of Oviedo founded in 2014 that provides a wide range of services and products in the nanobiotechnology field.

Products

  • Nanoparticles
  • Pronanosomes

Latest news

  • LIPOSOMES FOR BRAIN DRUG DELIVERYJanuary 29, 2020 - 15:32
  • Liposomes and PLGA nanoparticles as carriers for vaccines.December 2, 2019 - 17:06
  • GOLD NANOMATERIALS APPLICATIONS: THE ROLE OF THE SHAPE (NANORODS AND NANOSTARS)March 22, 2019 - 12:07
© Copyright - Nanovex Biotechnologies SL | Desarrollo web por Logic Think
  • Twitter
  • LinkedIn
  • Legal notice
  • Política de calidad
  • Cookies
Transfectosome Exosome characterization
Scroll to top
Our website uses cookies to help provide you with the best experience. You can consult more information about our cookies policy. I agree Reject Read more
Privacy & Cookies Policy

Privacy Overview

This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may affect your browsing experience.
Necessary
Always Enabled

Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.

Non-necessary

Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.